Cloning and expression of lipase gene from Ralstonia eutropha in Escherichia coli
نویسندگان
چکیده
Background Lipases are enzymes belonging to the class of hydrolases, acting in the aqueous-organic interface demonstrating considerable levels of activity and stability in aqueous environments and non-aqueous [1]. Its biological function is to catalyze the hydrolysis of long chain triglycerides to form fatty acids and glycerol, in addition to performing esterification [2]. They have wide industrial applicability and can be used in the composition of detergents, in food industry, in wastewater treatment and fine chemistry [3]. The goal this work was clone and express lipase gene from Rasltonia eutropha in Escherichia coli BL21DE3.
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